Quantitative Fluorescence Imaging for Investigation and Treatment of Disease

Author

Lemoyne Michael Habimana-Griffin, Washington University in St. LouisFollow

ORCID

http://orcid.org/0000-0003-1138-6976

Date of Award

Spring 5-15-2020

Author's School

McKelvey School of Engineering

Author's Department

Biomedical Engineering

Degree Name

Doctor of Philosophy (PhD)

Degree Type

Dissertation

Abstract

Molecular imaging is a powerful tool that enables interrogation of basic molecular mechanisms, diagnosis of disease, guidance of therapeutic modalities and monitoring of treatment response. Among the various imaging modalities, optical imaging is particularly suited for preclinical molecular imaging owing to its high sensitivity, lack of exposure to ionizing radiation, low cost, portability and scalability of imaging from the microscopic to macroscopic scale. In particular, fluorescence molecular tomography (FMT) provides quantitative 3D reconstructions of fluorescence distributions down to picomole quantities allowing for whole animal molecular imaging. In this work, FMT is applied to detect disease-specific molecular probes, to monitor and quantify delivery of therapeutic drugs and to understand basic mechanisms of disease, with an emphasis on imaging uptake and clearance of molecular probes in the brain.

Language

English (en)

Chair

Samuel Achilefu

Committee Members

Mark Anastasio, Dennis Callahan, Monica Shokeen, Christina Tsien,

Comments

Permanent URL: https://doi.org/10.7936/465d-wd10