Simulation Of The Undiseased Human Cardiac Ventricular Action Potential: Model Formulation, Experimental Validation And Application

Author

Thomas O'Hara, Washington University in St. LouisFollow

Author's School

School of Engineering & Applied Science

Author's Department/Program

Biomedical Engineering

Language

English (en)

Date of Award

5-24-2011

Degree Type

Dissertation

Degree Name

Doctor of Philosophy (PhD)

Chair and Committee

Yoram Rudy

Abstract

Creation of an accurate model for the undiseased human ventricular action potential: AP) which reproduces a broad range of physiological behaviors requires extensive experimental data, of which essential elements have been unavailable. We developed and validated such a model using new experimental data from endocardium of 140 undiseased human hearts. 78 were from male: 56%). Average age was 41 with standard deviation of 12 years. New experiments include: Ca²⁺ versus voltage dependent inactivation of L-type Ca²⁺ current; kinetics for the transient outward, rapid delayed rectifier, Na⁺/Ca²⁺ exchange, and inward rectifier currents; AP recordings at all physiological cycle lengths; steady state rate dependence and restitution of AP duration. The model was used to investigate the "silent" Long-QT mutation Q357R in KCNQ1.

Comments

Permanent URL: http://dx.doi.org/10.7936/K70R9MCM

Recommended Citation

O'Hara, Thomas, "Simulation Of The Undiseased Human Cardiac Ventricular Action Potential: Model Formulation, Experimental Validation And Application" (2011). All Theses and Dissertations (ETDs). 873.
https://openscholarship.wustl.edu/etd/873