Document Type
Conference Proceeding
Publication Date
2-22-2019
Abstract
Various methods exist for measuring molecular orientation, thereby providing insight into biochemical activities at nanoscale. Since fluorescence intensity and not electric field is detected, these methods are limited to measuring even-order moments of molecular orientation. However, any measurement noise, for example photon shot noise, will result in nonzero measurements of any of these even-order moments, thereby causing rotationally-free molecules to appear to be partially constrained. Here, we build a model to quantify measurement errors in rotational mobility. Our theoretical framework enables scientists to choose the optimal single-molecule orientation measurement technique for any desired measurement accuracy and photon budget.
Recommended Citation
Zhang, Oumeng and Lew, Matthew D., "Fundamental limits of measuring single-molecule rotational mobility" (2019). Electrical & Systems Engineering Publications and Presentations. 5.
https://openscholarship.wustl.edu/ese_facpubs/5
Included in
Biological and Chemical Physics Commons, Electrical and Computer Engineering Commons, Optics Commons, Physical Chemistry Commons
Comments
Copyright 2019 Society of Photo‑Optical Instrumentation Engineers (SPIE). One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this publication for a fee or for commercial purposes, and modification of the contents of the publication are prohibited.
Oumeng Zhang, Matthew D. Lew, "Fundamental limits of measuring single-molecule rotational mobility," Proc. SPIE 10884, Single Molecule Spectroscopy and Superresolution Imaging XII, 1088412 (22 February 2019); doi: 10.1117/12.2506712