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Document Type
Article
Language
English (en)
Publication Date
9-1-2025
Abstract
Single-molecule localization microscopy (SMLM) offers enhanced spatial resolution in optical microscopy, providing detailed insights into the spatial organization of proteins in cells at the nanoscale. Over the past decade, SMLM has progressively incorporated the capability to retrieve the orientations of single molecules using their polarized dipolar emission pattern. Here we explore recent advancements in single-molecule orientation and localization microscopy (SMOLM), which yields super-resolved images of molecular three-dimensional (3D) orientations, wobble and 3D positions. This advancement opens possibilities to explore the nanoscale organization and conformation of biological molecules as well as to monitor and design local 3D optical fields in nanophotonics. We cover the principles of SMOLM, discuss recent advances and applications in biology and photonics, and finally highlight exciting future directions and challenges in the field.
Recommended Citation
Brasselet, Sophie and Lew, Matthew D., "Single-molecule orientation and localization microscopy" (2025). Electrical & Systems Engineering Publications and Presentations. 21.
https://openscholarship.wustl.edu/ese_facpubs/21
Included in
Atomic, Molecular and Optical Physics Commons, Bioimaging and Biomedical Optics Commons, Biological and Chemical Physics Commons, Electromagnetics and Photonics Commons, Nanoscience and Nanotechnology Commons, Optics Commons
Comments
This article is the accepted version of the manuscript available below:
Brasselet, S., Lew, M.D. Single-molecule orientation and localization microscopy. Nat. Photon. 19, 925–937 (2025). https://doi.org/10.1038/s41566-025-01724-y