Abstract
Antibody-antigen recognition enables antibody-conjugated nanostructures to serve as plasmonic biosensors with tunable specificity. However due to the instability of antibodies, these biosensors are susceptible to changes in the environment such as heat and aridity, leading to constraints on the transportation and handling of these sensors. Here we establish a method using a metal-organic framework crystal to preserve biosensor activity under severe environmental conditions, including exposure to high temperatures, an organic solvent and a proteolytic agent. After zeolitic imidazolate framework-8 (ZIF-8) crystals formed for 12 hours on a biosensor of gold nanorods conjugated with a model antibody, rabbit IgG, 80% of the antibody activity is successfully retained after incubation at 60 °C for 24 hours. We investigated the change of preservation ability with respect to the growth of ZIF-8 film. Furthermore, in comparison with silk fibroin films, which showed similar function in previous studies, ZIF-8 films demonstrate stronger preservation ability in various conditions.
Committee Chair
Srikanth Singamaneni
Committee Members
Srikanth Singamaneni, Guy M. Genin, Jeremiah J. Morrissey
Degree
Master of Science (MS)
Author's Department
Mechanical Engineering & Materials Science
Document Type
Thesis
Date of Award
Fall 12-21-2016
Language
English (en)
DOI
https://doi.org/10.7936/K7VM49PD
Recommended Citation
Wang, Lu, "Activity Preservation of Plasmonic Biosensors with a Metal-Organic Framework" (2016). McKelvey School of Engineering Theses & Dissertations. 199.
The definitive version is available at https://doi.org/10.7936/K7VM49PD
Comments
Permanent URL: https://doi.org/10.7936/K7VM49PD