Technical Report Number
When using random clone overlap based methods to make DNA maps, fragment matching mistakes, the incorrect matching of similar length restriction fragments, are a common problem that produces incorrect maps. This technical report discusses techniques for fragment splitting and fragment combining, which attempts to correct maps containing a fragment matching mistake, given that the location of the mistake is known. These techniques are based on operations that decompose and merge virtual fragments (a collection of matched real fragments), add virtual fragments to existing groups in the map and insert virtual fragments between existing groups in the map. Examples of applying these techniques to both contrived and actual DNA maps are presented.
Daues, James and Gillet, Will, "DNA Mapping Algorithms: Fragment Splitting and Combining" Report Number: WUCS-91-50 (1991). All Computer Science and Engineering Research.