Basecalling for Traces Derived for Multiple Templates

Authors

Aaron Tenney, Washington University in St. Louis

Document Type

Technical Report

Publication Date

2004-12-10

Filename

wucse-2004-80.pdf

Technical Report Number

WUCSE-2004-80

Abstract

Three methods for analyzing sequencing traces derived from sequencing reactions containing two DNA templates are presented. All rely on alignment to a segment of assembled genomic sequence containing the original template sequence. Spliced alignment algorithms are used so that traces derived from processed mRNA can be analyzed. The main application of these techniques is the elucidation of alternately spliced transcripts. Several experimental verification of one of the techniques is presented including testing on a set of 48 alternately spliced targets from the human genome and 47 negative controls.

Comments

Permanent URL: http://dx.doi.org/10.7936/K7QZ28BZ

Recommended Citation

Tenney, Aaron, "Basecalling for Traces Derived for Multiple Templates" Report Number: WUCSE-2004-80 (2004). All Computer Science and Engineering Research.
https://openscholarship.wustl.edu/cse_research/1051