Document Type
Book Chapter
Publication Date
2010
Originally Published In
Methods Cell Biol. 2010;95:521-42. doi: 10.1016/S0091-679X(10)95026-7.
Abstract
Microtubules are part of a complex mechano-chemical network inside cells. In order to understand how the components of these systems work together, careful in vitro experiments must be performed with added complexity. These experiments can ideally image all the interacting species. In order to image these molecules, multiple-color fluorescence imaging can be performed. In this chapter, we describe some methods for performing multiple-color single molecule fluorescence imaging using total internal reflection fluorescence microscopy. We give several specific examples of species of microtubule-associate proteins and motors that can be examined with detailed protocols for labeling, purification, and imaging.
Recommended Citation
Ross, Jennifer L. and Dixit, Ram, "Chapter 26 – Multiple Color Single Molecule TIRF Imaging and Tracking of MAPs and Motors" (2010). Biology Faculty Publications & Presentations. 74.
https://openscholarship.wustl.edu/bio_facpubs/74
Comments
Final author manuscript version of article published Methods Cell Biol. 2010;95:521-42. doi: 10.1016/S0091-679X(10)95026-7. © 2010 Elsevier