ORCID
http://orcid.org/0000-0002-3600-8157
Date of Award
Spring 5-15-2021
Degree Name
Doctor of Philosophy (PhD)
Degree Type
Dissertation
Abstract
Most biological processes are associated with protein catalysis. Characterizing protein structure, therefore, is crucial for understanding biological function. Mass spectrometry (MS)-based methods have emerged as a pivotal biophysical tool to interrogate protein structures; they can characterize protein-ligand/protein complexes that are refractory to conventional high-resolution means such as X-ray crystallography, Cryo-EM, and nuclear magnetic resonance (NMR) spectroscopy, in part owing to high molecular weight and significant flexibility. The integrated MS-based platform will provide topology and structural information of proteins from sketch to detail; native MS identifies protein complexes’ composition and stoichiometry; hydrogen-deuterium exchange MS (HDX-MS) detects the protein complexes’ dynamic change at the peptide-level and sometimes at the residue-level; protein footprinting coupled with MS pinpoints the binding site at residue-level via irreversible chemical changes. This thesis comprising eight chapters focuses on method development and application of integrated MS-based platform, using examples of metal-binding proteins and integral membrane proteins that illustrate challenges and solutions.
Language
English (en)
Chair and Committee
Michael L. Gross
Committee Members
Jeffrey P. Henderson, John-Stephen Taylor, Meredith Jackrel, Timothy Wencewicz,
Recommended Citation
Guo, Chunyang, "Integrated Mass Spectrometry-Based Method in Protein Chemistry: Metal-binding Protein and Integral Membrane Protein" (2021). Arts & Sciences Electronic Theses and Dissertations. 2420.
https://openscholarship.wustl.edu/art_sci_etds/2420