Lateral Inhibition Shapes Neuronal Selectivity for Natural Stimuli in Mouse Accessory Olfactory Bulb

Date of Award

Spring 5-15-2010

Author's School

Graduate School of Arts and Sciences

Author's Department

Biology & Biomedical Sciences (Neurosciences)

Degree Name

Doctor of Philosophy (PhD)

Degree Type

Dissertation

Abstract

Laterally connected inhibitory circuitry is found throughout the nervous system, including many early sensory processing systems. The extent to which it plays a role in shaping neuronal stimulus selectivity in systems like olfaction, however, which lack a simple two-dimensional representation of their stimulus space, has remained controversial. We examined this issue using a novel experimental preparation that allowed electrophysiological recording from the accessory olfactory bulb of an anesthetized mouse during the controlled delivery of pheromonal stimuli, in this case derived from the urine of male and female mice. We found that individual neurons were often highly selective for the sex of the urine donor. Examination of both explicitly inhibitory responses, as well as responses to mixtures of male and female urine, revealed that laterally connected inhibition was both prevalent and of large magnitude. Pharmacological manipulation of this inhibition resulted in a shift in many neurons’ stimulus selectivities. Finally, we found that a behavioral response (pregnancy block) evoked by the presence of unfamiliar male urine could be suppressed by the addition of female urine to the stimulus, demonstrating that this system displays a behavioral opponency consistent with neural inhibition. Together, these results indicate that laterally connected inhibitory circuitry in the AOB plays an important role in shaping neural selectivity for natural stimuli.

Language

English (en)

Chair and Committee

Timothy E. Holy

Committee Members

Dora E. Angelaki, Paul A. Gray, Steven J. Mennerick, Lawrence H. Snyder, Kurt A. Thoroughman

Comments

Permanent URL: https://doi.org/10.7936/K7RR1W6G

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