Enzymes are naturally occuring sustainable catalysts that offer competitive catalysis to synthetic routes. There exist growing demands for environmentally-friendly and sustainable catalysis, which have led to a surge of research in biocatalysis. 1-3 However, the major bottleneck for the industrial applications of enzymes is the long term operational stability and difficultly in recovery and reusability of enzymes. 4-6 The goal of this study was to develop biocatalytic surface with organo-silica enzyme imprints that can recover, stabilize and reuse enzymes. The complete encapsulation was expected to enhance thermal and chemical stability, and the plasmonic nanostructures were expected to provide the ability to enhance the biocatalytic activity when light was used as an external trigger. The target enzyme was horseradish peroxidase (HRP).
Mechanical Engineering and Material Sciences Independent Study
Date of Submission
Yim, Jieun and Singamaneni, Srikanth, "Organosilica Imprints for Preserving and Enhancing Biocatalytic Activity of Immobilized Enzyme" (2016). Mechanical Engineering and Materials Science Independent Study. 25.