Date of Award
Doctor of Philosophy (PhD)
Chair and Committee
Peptide Nucleic Acids: PNAs), are an ideal choice for antisense and antigene tools and probes because of their stability and high binding affinity, but have limited application as therapeutic and diagnostic agents because of poor membrane permeability. To increase membrane permeability, we conjugated hydrophobic lipids or phospholipid molecules and hydrophilic cell penetrating peptides to the N and C terminus of PNAs, and studied their physical and biological properties. After solid-phase automated synthesis, PNA-CPPs: TAT/Arg9) were coupled with lipids or phospholipids before cleavage from the support and then purified by HPLC. The conjugates were characterized by UV-vis and MALDI, and by their ability to form vesicles in water and to partition between water and octanol. The size of the vesicles formed was determined by DLS. One of the Lipid-PNA-TAT conjugates was further tested in vivo as a potential PET imaging reagent after radiolabeling with bromine-76.
Song, Yinyin, "Design, Synthesis And Characterization Of Lipidated Pna-Peptide Conjugates As Potentialtherapeutic And Diagnostic Reagents" (2011). All Theses and Dissertations (ETDs). 643.