Date of Award
Master of Science (MS)
Angiogenesis is one of the most challenging problems in bone structure repair. The different types of 3D fibrous morphology structure of the extracellular matrix for fasting angiogenesis have been studied. Due to the densely packed constructs and limited porosity of scaffold, the challenge still remains for scaffold fabrication. In this study, we fabricated PCL scaffold with growth factors and cells to guide fast angiogenesis by electro-spinning and electro-spray simultaneously. We developed a technique that electro-spinning encapsulates growth factors OPN(Recombinant Human Osteopontin) and SDF-1α(CXCL12) in poly(lactide-co-glycolide) (PLGA) microspheres into PCL 3D structures scaffold fibers. We also developed a new technique to fabricated cells into PCL scaffold coating with gelatin A. And tested release amount of growth factors in scaffold over 28 days and loading efficiency. We can prove that microsphere released growth factors successfully into release medium in vitro. The Young’s module and elongation rate been tested by the single axis tensile test. The migration in collagen gel and in fibers were tested and quantified by confocal fluorescence microscopy. In vitro cell migration study demonstrated that OPN and SDF-1 significantly increased the depth of cell migration. In vivo, the scaffold with growth factors improve the repair of bone fracture in RA mice. Based on the results, these 3D scaffold may have potential to engineer tissues for various applications.