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Optical and Multimodal Imaging of Cancer Assisted by Fluorescence Goggle and Microscopy
Date of Award
Doctor of Philosophy (PhD)
Current surgical oncology faces great challenges due to the lack of accurate intraoperative image-guidance and rapid histopathological assessment. This leads to incomplete tumor resections and cancer recurrences. We have developed the fluorescence goggle system to overcome these limitations. The fluorescence goggle is a real-time intraoperative imaging and display system that, when combined with fluorescent molecular probes, can identify the extension of tumors and guide surgical resections. It is compact, wireless, wearable, battery-operated, and allows for hands-free imaging by surgeons. Unlike conventional imaging instruments, the fluorescence goggle directly displays fluorescence information on its eyepieces. We have developed several generations of fluorescence goggle based on CCD and CMOS imaging technologies, as well as see-through display technologies. We have applied the fluorescence goggle to aid cancer staging and surgical resection of breast, liver and brain cancer, in both small animals and humans. To facilitate pathological diagnosis, we have developed a complementary fluorescence-polarization dual-modal microscope and a division of focal plane polarized light microscope. These novel microscopes greatly assist rapid histopathological assessment of cancer. The combination of real-time image-guided surgeries using the fluorescence goggle and ex vivo histopathology using the multimodal microscopes improves surgical planning and patient outcomes.
Gregory Lanza, Barbara Monsees, Lihong Wang, Samuel Achilefu