Date of Award

Spring 5-7-2024

Author's School

McKelvey School of Engineering

Author's Department

Biomedical Engineering

Degree Name

Master of Science (MS)

Degree Type



This research investigates the design and application of phase-separating peptides for direct cytosolic drug delivery. Novel peptides (GHGXY)4, with variable amino acids, were synthesized using microwave-assisted chemistry to enhance phase separation and membrane fusion. In vitro experiments utilizing advanced imaging techniques demonstrated the peptides' capability to form coacervates and facilitate direct cargo, DNA, mRNA, and protein, delivery into the cytosol, bypassing traditional cellular barriers. The study also explores the role of inhibitor Bafilomycin and NH4Cl in phase-separating peptides functionality, offering significant insights into their delivery mechanisms. This research underscores the potential of phase-separating peptides in improving therapeutic delivery strategies.


English (en)


Jai Rudra

Committee Members

Jai Rudra Meredith Jackrel Michael Vahey

Available for download on Wednesday, May 07, 2025