Tissue Engineering a 3D In Vitro of Perfused Human Cardiac Muscle
Tissue engineering holds enormous potential to not only replace or restore function to a wide range of tissues, but also to capture and control 3D physiology in vitro (e.g., microphysiological systems). The latter has important applications in the fields of drug development, toxicity screening, and repairing damaged cardiac (heart) muscle. In order to replicate the complex 3D arrangement of cells and extracellular matrix (ECM), new human microphysiological systems must be developed, and must include a vascular supply. The vasculature not only provides the necessary convective transport of nutrients and waste in 3D culture, it also couples and integrates the response of multiple organ systems. The past decade has brought tremendous advances in our understanding of pluripotent stem cells, blood vessel formation, and microfabrication, providing a rich environment to design new microphysiological systems. This seminar will describe our approach and early results to create 3D human cardiac muscle perfused with human capillaries, all from the same source of induced pluripotent stem cells.
Professor & Chair, Department of Biomedical Engineering Washington University in St. Louis