An important goal of the Cardiac Bioelectricity and Arrhythmia Center (CBAC), Washington University in St. Louis is to enhance and promote education and training in biomedical engineering, life sciences, and clinical medicine. The CBAC hosts seminars each semester, where world renowned clinicians and researchers are invited to lecture. These seminars are attended by engineering, physics and medical school faculty and graduate students, as well as professionals from outside the university. These seminar videos are open to the general public.
Disclaimer: All material within each video file was filmed during a Cardiac Bioelectricity and Arrhythmia Center (CBAC) seminar at Washington University in St. Louis. It was made available on this website with permission from the speaker, who reserves and holds all rights. The video files are for educational purposes only and may not be reproduced, distributed or copied in any format.
Videos will load in an external platform. For full screen viewing, click on the bottom right corner of the video screen.
For more information about the seminars, please contact Huyen (Gwen) Nguyen at hbnguyen@wustl.edu
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Ventricular Tachycardia Following Myocardial Infarction: What is the Substrate?
Alfred E. Buxton M.D.
2-5-2018
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Treatment of Ventricular Arrhythmias in Structural Heart Disease
David J. Callans M.D., F.H.R.S.
4-16-2018
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Using Genetics to Identify the Molecular Mechanisms of Atrial Fibrillation
Patrick T. Elinor M.D., Ph.D
12-18-2017
Atrial fibrillation (AF) affects over 3 million individuals in the US and 4.5 million individuals in Europe. In addition, AF is associated with an increased risk of stroke, dementia, heart failure, death and health care costs. Many AF risk factors have been identified including aging, cardiovascular disease, and family history. Although heritable forms of AF were previously considered rare, we have established evidence of substantial AF heritability over the last decade. Genome-wide association studies (GWAS) provide a powerful tool to identify common variants underlying disease risk. In 2007 a GWAS for AF identified a locus on chromosome 4q25. Later that ...Read More
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Massively-Parallel All-Optical Cardiac Electrophysiology
Emilia Entcheva Ph.D.
4-17-2017
Optical targeting (stimulation or recording) allows distributed parallel access to thousands and even millions of cells and locations at the same time, and within the tissue setting; optical targeting is high-throughput by nature. In this talk, I will discuss and demonstrate the combination of optogenetic stimulation with optical imaging of electrical activity in cardiomyocytes, i.e. the realization of “all-optical electrophysiology” in a high-throughput manner (HTS). Examples will include demonstration of cardiac “wave steering” by light and the use of a new fully-automated all-optical HTS platform (OptoDyCE) for drug screening using patient-derived cardiomyocytes (iPS-CMs).
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Novel Regulators of Cardiac Rhythm
Glenn I. Fishman M.D., F.A.C.C., F.A.H.A., F.A.C.P., F.H.R.S
12-4-2017
Our laboratory has been interested in multiple facets of cardiac electrophysiology and arrhythmia mechanisms, with a particular focus on identifying novel regulators of cardiac excitability, impulse propagation, and arrhythmia susceptibility. Our most recent studies seek to decipher the mechanisms responsible for specification, maturation and function of the specialized cardiac conduction system and identifying novel transcriptional regulators that influence cardiomyocyte electrophysiological diversity and integrated cardiac excitability. I will describe the role of two novel transcription factors (one recently reported and the second still a work in progress) that appear to play critical roles in establishing functional diversity throughout individual compartments of ...Read More
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Membrane-mediated Alterations in Cardiac Bioenergetics, Electrophysiology & Signaling
Richard W. Gross M.D., Ph.D
11-27-2017
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Lessons Learned from Mapping & Ablation of Nonischemic Cardiomyopathy
Francis E. Marchlinski M.D.
11-20-2017
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Cardiac Mechanoelectric Feedback: A Role for Caveolae?
Andrew D. McCulloch Ph.D.
1-18-2017
Mechanical stretch of cardiac muscle affects myocyte electrophysiology is potentially arrhythmogenic. We investigated the effects of stretch in vitro and in the intact heart on action potential conduction. Using optical mapping to measure in isolated mouse hearts and in micropatterned mouse cardiomyocyte we found that conduction velocity decreased rapidly and reversibly with volume loading and stretch. This slowing was not altered by stretch-activated channel inhibition, but was abrogated when caveolae were disrupted by genetic deletion of caveolin-3 or chemical depletion of membrane cholesterol. Electron microscopy showed that stretch in wild-type mouse hearts, causes recruitment of caveolae to the sarcolemma. Stretch ...Read More
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Defining New Pathways Underlying Human Arrhythmia
Peter J. Mohler Ph.D.
3-6-2017
Our research is focused on the mechanisms underlying the targeting and regulation of membrane-associated (ion channels, transporters, receptors) and signaling proteins in cardiac and other excitable cells. In particular, we are interested in the role of membrane-associated ankyrin and spectrin family of polypeptides in the targeting and function of ion channels and transporters as well as kinases and phosphatases. A primary focus of the lab is the role of the ankyrin-G-based pathway for targeting voltage gated sodium channels to the intercalated disc of cardiomyocytes. We have discovered a direct requirement of ankyrin-G for Na channel targeting and have linked human ...Read More
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Advances in Cardiac Resynchronization Therapy – Have We Maximized Electrical Resynchrony?
Amit Noheria M.B.B.S. (M.D.), S.M.
1-23-2017
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Investigating Variability in Human Electrophysiology: Population, Drugs and Disease
Blanca Rodriguez Ph.D.
2-3-2017
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Longer Lasting Torsade de Pointes Arrhythmias Require Reentry for its Maintenance in the CAVB Dog Model
Marc A. Vos Ph.D.
5-8-2017
The mechanism of Torsade de Pointes arrhythmias (TdP) has been under debate for many years. Focal activity as well as reentry have both been mentioned in the intiation as perpetuation of TdP. Therefore, the arrhythmogenic mechanisms responsible for TdP were once more investigated in the CAVB dog model, known for its high susceptibility for TdP. In five TdP-sensitive CAVB dogs, 56 needle electrodes were evenly distributed transmurally to record 240 unipolar local electrograms simultaneously. Nonterminating (NT) episodes were defibrillated after 10s. Software was developed to automatically detect activation times and to create 3D visualisations of the arrhythmia. For each episode ...Read More
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K+ Channel – Linked Arrhythmias: Lessons from a Decade of Studying KCNE Knockout Mice
Geoffrey W. Abbott Ph.D.
2-15-2016
Coronary Artery Disease (CAD) leads to more deaths in the U.S. and globally each year than any other single cause of death. An estimated half of CAD cases involve genetic predisposition; reduction of other risk factors can reduce CAD mortality and morbidity by >30%. The major challenge now is to develop more comprehensive prevention and treatment strategies for both genetic and environmental risk factors, necessitating a better mechanistic understanding of CAD. Another form of fatal heart disorder, Sudden Cardiac Death (SCD), accounts for ~1000 deaths per day in the U.S. SCD is thought to require an electric substrate, an ischemic ...Read More
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Cardiac Sodium Channel Nav1.5 & Interacting Proteins: Roles in Cardiac Channelopathies
Hugues Abriel M.D., Ph.D.
5-2-2016
The field of molecular arrhythmology has progressed at an impressive pace during the past 20 years. Throughout the years, we have learned more and more about the genetic factors and molecular mechanisms underlying electrical abnormalities of the heart such as congenital long QT syndrome (LQTS) and Brugada syndrome. Since in most cases, the genes that are found to be mutated are encoding either the pore-forming subunit of cardiac ion channels or of ion channel regulatory proteins, the term “genetic cardiac channelopathies” has been used to define these disorders. Among the still-growing list of genes that lead to genetic cardiac channelopathies, ...Read More
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Atrial Remodeling Induced by Exercise: a Model(s) for Atrial Fibrillation
Peter H. Backx Ph.D., D.V.M.
1-25-2016
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Regulation of Cardiac Calcium Channels in the Fight-or-Flight Response: From Molecular Mechanisms to Heart Failure
William Catterall Ph.D.
11-21-2016
Intracellular calcium transients generated by activation of voltage-gated calcium (CaV) channels generate local signals, which initiate physiological processes such as secretion, synaptic transmission, and excitation-contraction coupling. Regulation of calcium entry through CaV channels is crucial for control of these physiological processes. In this article, I review experimental results that have emerged over several years showing that cardiac CaV1.2 channels form a local signaling complex, in which their proteolytically processed distal C-terminal domain, an A-Kinase Anchoring Protein, and cyclic AMP-dependent protein kinase (PKA) interact directly with the transmembrane core of the ion channel through the proximal C-terminal domain. This signaling complex ...Read More
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Development of Entirely Noninivasive Cardiac Ablation Platform
Phillip S. Cuculich M.D. and Clifford G. Robinson M.D.
11-28-2016
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From Protein to Mouse to Human: cBIN1 in E-C Coupling and Heart Failure Management
TingTing Hong M.D.; Ph.D.
12-5-2016
The Hong Lab has cloned the cardiac isoform of the membrane deformation protein BIN1, which we refer to as cBIN1. The story of cBIN1 originated as a protein trafficking finding, identifying how calcium channels are delivered to T-tubule membrane. In subsequent studies, they have found that cBIN1 forms membrane microdomains in T-tubule membrane, affecting calcium transients, dyad organization, and membrane excitability. In heart failure, cBIN1 is reduced. cBIN1 is also turned over into blood from viable cardiomyocytes. In clinical studies, they are finding that cBIN1 provides a window into the metabolic health of heart muscle and may help in clinical decision making.
