Originally Published In
Egelhofer TA, Minoda A, Klugman S, et al. An assessment of histone-modification antibody quality. Nat Struct Mol Biol. 2011;18(1):91–93. doi:10.1038/nsmb.1972
We have tested the specificity and utility of more than 200 antibodies raised against 57 different histone modifications in Drosophila melanogaster, Caenorhabditis elegans and human cells. Although most antibodies performed well, more than 25% failed specificity tests by dot blot or western blot. Among specific antibodies, more than 20% failed in chromatin immunoprecipitation experiments. We advise rigorous testing of histone-modification antibodies before use, and we provide a website for posting new test results (http://compbio.med.harvard.edu/antibodies/).
Egelhofer, Thea; Minoda, Aki; Klugman, Sarit; Lee, Kyungjoon; Kolasinska-Zwierz, Paulina; Alekseyenko, Artyom; Cheung, Ming-Sin; Day, Daniel; Gadel, Sarah; Gorchakov, Andrey; Gu, Tingting; Kharchenko, Peter; Kuan, Samantha; Latorre, Isabel; Linder-Basso, Daniela; Luu, Ying; Ngo, Queminh; Perry, Marc; Rechtsteiner, Andreas; Riddle, Nicole; Schwartz, Yuri; Shanower, Gregory; Vielle, Anne; Ahringer, Julie; Elgin, Sarah C.R.; Kuroda, Mitzi; Pirrotta, Vincenzo; Ren, Bing; Strome, Susan; Park, Peter; Karpen, Gary; Hawkins, R; and Lieb, Jason, "An assessment of histone-modification antibody quality" (2011). Biology Faculty Publications & Presentations. 238.