Author's School

Arts & Sciences

Author's Department

Biology

Document Type

Article

Publication Date

1-2011

Originally Published In

Egelhofer TA, Minoda A, Klugman S, et al. An assessment of histone-modification antibody quality. Nat Struct Mol Biol. 2011;18(1):91–93. doi:10.1038/nsmb.1972

Abstract

We have tested the specificity and utility of more than 200 antibodies raised against 57 different histone modifications in Drosophila melanogaster, Caenorhabditis elegans and human cells. Although most antibodies performed well, more than 25% failed specificity tests by dot blot or western blot. Among specific antibodies, more than 20% failed in chromatin immunoprecipitation experiments. We advise rigorous testing of histone-modification antibodies before use, and we provide a website for posting new test results (http://compbio.med.harvard.edu/antibodies/).

Comments

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ORCID

https://orcid.org/0000-0002-5176-2510 [Elgin]

DOI

10.1038/nsmb.1972

Included in

Biology Commons

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