Date of Award
Doctor of Philosophy (PhD)
The reaction center (RC) complex of the green sulfur bacterium Chlorobaculum tepidum is composed of the Fenna-Matthews-Olson (FMO) antenna protein and the reaction center core (RCC) complex. The RCC complex has four subunits: PscA, PscB, PscC, and PscD. The structure of the intact and functional FMO-RCC complex was studied by chemically cross-linking the purified sample followed by biochemical and spectroscopic analysis. The interaction sites of the cross-linked complex were also studied using LC-MS/MS. A structural model is proposed based on those results. In addition, the RCC complexes were purified, both the PscA-PscC complex from the Chlorobaculum tepidum and the PscA-PscB complex from Prosthecochloris aestuarii. The intact FMO-RCC complex and the RCC complexes were further studied comparatively by steady-state and time-resolved fluorescence and femtosecond time-resolved transient absorption spectroscopies to elucidate the pathway of FMO-to-RCC inter-protein energy transfer as well as RCC intra-protein energy and electron transfer.
Chair and Committee
Robert E Blankenship
Dewey Holten, Michael L Gross, Timothy Wencewicz, Liviu Mirica, Joseph Jez,
He, Guannan, "Structural and Functional Analysis of the Reaction Center Complexes from the Photosynthetic Green Sulfur Bacteria" (2015). Arts & Sciences Electronic Theses and Dissertations. 554.