Date of Award

Winter 12-15-2022

Author's School

Graduate School of Arts and Sciences

Author's Department

Biology & Biomedical Sciences (Molecular Genetics & Genomics)

Degree Name

Doctor of Philosophy (PhD)

Degree Type



Humans are incapable of complex body structure regeneration. Understanding how other species retain the ability to regenerate can help us to find solutions for improving our regenerative capacities. The zebrafish is an organism that is capable of regenerating multiple organs in its body. Using zebrafish as a model organism, previous studies have identified key processes and illustrated the crucial cell types and genes for regeneration. In caudal fin specifically, blastema formation is a key predictor of successful regeneration. However, a comprehensive molecular understanding of cell type-specific contributions to this process is lacking. In this dissertation, I present our efforts toward providing a holistic view of blastema formation in zebrafish caudal fin regeneration. Using single-cell transcriptome and epigenome profiling, I charted regeneration response in multiple cell types and found a putative regulatory network guiding cell state transition and cell-cell interactions during the blastema formation process. This work provides a single-cell level perspective to the general understanding of regeneration and serves as a resource for functional characterization targeting key genes and regulatory genomic regions in individual populations involved in regeneration responses.



English (en)

Chair and Committee

Ting Wang

Committee Members

John Edwards


Update embargo

Available for download on Friday, August 09, 2024

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Biology Commons