Date of Award
Doctor of Philosophy (PhD)
Microelectrode arrays contain collections of spatially isolated electrodes that can be individually addressed. Thus, the arrays have the potential to support synthesis of molecular libraries if unique members of the library can be located next to unique electrodes in the array. The result would be an intriguing platform for screening the molecules in the library against biological receptors in order to identify high-affinity ligands for those targets, especially since the arrays themselves can be directly used in the signaling studies. To expand the capabilities of the arrays, the goal of this thesis is to develop the new array chemistry needed to build a larger library. Our efforts in this area started with improving the surface coating on the array that supports every synthetic and analytic experiment conducted on the arrays. Once we had an optimized surface for performing chemistry on the arrays, I investigated protecting group strategies for parallel synthesis on the arrays. This eventually led me to develop new chemistry for diversifying molecules on the array that also facilitates characterization of the array-bound substrates. This new chemistry will help us to more fully utilize the bioanalytical potential of the arrays.
Chair and Committee
Jonathan Barnes, John-Stephen Taylor, Timothy Wencewicz, Zhen He,
Yeh, Nai-Hua, "Developing Methods for Diversifying Molecular Scaffolds Directly on a Microelectrode Array" (2021). Arts & Sciences Electronic Theses and Dissertations. 2386.