This item is under embargo and not available online per the author's request. For access information, please visit http://libanswers.wustl.edu/faq/5640.

ORCID

http://orcid.org/0000-0002-4172-3681

Date of Award

Summer 8-15-2019

Author's School

Graduate School of Arts and Sciences

Author's Department

Biology & Biomedical Sciences (Immunology)

Degree Name

Doctor of Philosophy (PhD)

Degree Type

Dissertation

Abstract

IgA is the primary antibody response at mucosal surfaces and is reported to inhibit adaptive immune responses against gut bacteria. Here, we utilize an in vitro system to expand and screen IgA memory B cells for their ability to recognize gut bacteria in the context of secretory IgA (sIgA) deficiency in polymeric Ig receptor (Pigr–/–) mice. Contrary to the prevailing hypothesis that IgA provides an immune exclusionary function, we found that mice lacking sIgA showed decreased anti-bacterial IgA specificities as assessed using flow cytometry. IgA B cell responses against certain taxa such as those of order Bacteriodales showed greater dependence on sIgA. Notably, sIgA also facilitated the generation of anti-bacterial IgG B cells, which provided increased resistance to intraperitoneal infection by commensal bacteria. Together, these data suggest that sIgA can facilitate the immune priming of gut commensals to increase anti-bacterial IgA and IgG and enhance immunity.

Language

English (en)

Chair and Committee

Chyi S. Hsieh

Committee Members

Michael S. Diamond, Ali Ellebedy, Thaddeus S. Stappenbeck, Gregory F. Wu,

Available for download on Wednesday, November 04, 2020

Share

COinS