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Date of Award
Doctor of Philosophy (PhD)
Local translation in distal processes of the neuron was discovered over 35 years ago, but only with the recent advancements in microscopy, molecular techniques, and high throughput sequencing have we been able to identify which mRNAs are locally translated. Previous methods were unable to harvest cell specific RNA from neurites in vivo from a variety of cell types; however, building on these advancements, we developed SynapTRAP to examine localized translation in specific cell types. The diversity of arbors and functions of neurons across the central nervous system suggests different proteins localized to the periphery of cells for neurite growth and regulation, although it is unknown which of these proteins are synthesized locally. To determine if these different proteins are produced locally or if they are shuttled to the synapse post-translationally, we profiled two different cell types: GABAergic interneurons and layer V pyramidal neurons, using SynapTRAP. From these results we determined that not only do local translatomes differ between types of neurons, but that mRNA localization to neurites is regulated by both baseline cellular differences and post transcriptional pathways.
Chair and Committee
Joseph Karen . Dougherty O'Malley
Yehuda Ben-Shahar, Sergej Djuranovic, James Skeath,
Ouwenga, Rebecca, "Regulation of Local Translation in Neuronal Processes of Specific Cell Types" (2018). Arts & Sciences Electronic Theses and Dissertations. 1703.
Available for download on Thursday, December 15, 2118