Roles of Peroxisomes and Peroxisome-Derived Products in Controlling Plant Growth and Stress Responses
Date of Award
Doctor of Philosophy (PhD)
The peroxisome is a vital organelle conserved through the entire eukaryotic lineage. In all examined species, peroxisomes are responsible for such essential processes as fatty acid beta-oxidation and metabolism of reactive oxygen species (ROS). In plants, peroxisomes have taken on additional specialized roles, such as production of some plant hormones and vitamins. In this work, I have uncovered novel factors regulating peroxisome number in model species Arabidopsis thaliana, and novel mechanisms governing how peroxisomes respond to salt stress. I discovered a role for Arabidopsis MAP KINASE17 (MPK17) as a negative regulator of peroxisome division that acts in the salt-stress response pathway of peroxisome division. Additionally, I uncovered a novel role for the known peroxisome division factor PEROXISOME AND MITOCHONDRIAL DIVISION FACTOR 1 (PMD1) as another regulator of salt-induced peroxisome division and as the first known plant peroxisome division factor to bind to actin. A forward genetics approach was undertaken to attempt to isolate peroxisome-deficient mutants in the genetically tractable crop species Solanum lycopersicum, screening mutagenized tomato seeds on the auxin precursor indole-3-butyric acid (IBA). Although no peroxisome mutants were isolated by this method, several mutants impaired in various other aspects of auxin homeostasis were isolated and used to make new discoveries regarding the contributions of IBA to vegetative and reproductive tomato development.
Chair and Committee
Lucia C. Strader
Joseph Jez, Elizabeth Haswell, Bethany Zolman, Arpita Bose,
Frick, Elizabeth May, "Roles of Peroxisomes and Peroxisome-Derived Products in Controlling Plant Growth and Stress Responses" (2017). Arts & Sciences Electronic Theses and Dissertations. 1199.
Permanent URL: https://doi.org/10.7936/K75H7FPZ