Abstract

Transgenic and biochemical mouse models of osteoarthritis have become increasingly popular. However, the microscale thickness of mouse cartilage complicates mechanical characterization of these models. Existing methods are time-prohibitive and difficult, especially for life scientists. We developed a rapid, nondestructive method for viscoelastically characterizing murine articular cartilage, and validated it. We dissected tibial plateaus from adult mouse knees, and subjected them to trypsination and ribosylation treatments. Dynamic mechanical testing was performed using a BioDent testing apparatus (ActiveLife Scientific, Santa Barbara, CA). Viscoelastic parameters were extracted, and repeat testing was conducted to check for testing-induced damage. This protocol demonstrated significant mechanical differences between the control and osteoarthritic models. Additionally, we verified results using histology and finite element analysis.

Document Type

Final Report

Author's School

School of Engineering and Applied Science

Author's Department

Mechanical Engineering and Materials Science

Class Name

Mechanical Engineering and Material Sciences Independent Study

Date of Submission

5-16-2016

Available for download on Wednesday, May 15, 2019

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