Author's School

Arts & Sciences

Author's Department

Biology

Document Type

Article

Publication Date

5-20-2015

Originally Published In

Methods Mol Biol. 2015;1309:151-69. This also may be cited as Chapter 13 in Plant Gravitropism: Methods and Protocols. Editors: Elison B. Blancaflor. ISBN: 978-1-4939-2696-1 (Print) 978-1-4939-2697-8 (Online)

Abstract

The oocytes of the African clawed frog (Xenopus laevis) comprise one of the most widely used membrane protein expression systems. While frequently used for studies of transporters and ion channels, the application of this system to the study of mechanosensitive ion channels has been overlooked, perhaps due to a relative abundance of native expression systems. Recent advances, however, have illustrated the advantages of the oocyte system for studying plant and bacterial mechanosensitive channels. Here we describe in detail the methods used for heterologous expression and characterization of bacterial and plant mechanosensitive channels in Xenopus oocytes.

Comments

Final author version; the final publication is available at Springer via http://dx.doi.org/10.1007/978-1-4939-2697-8_13; © Springer Science+Business Media New York 2015.

DOI

10.1007/978-1-4939-2697-8_13

Embargo Period

5-20-2016

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