Date of Award
Spring 5-15-2016
Degree Name
Doctor of Philosophy (PhD)
Degree Type
Dissertation
Abstract
Group 1 ILCs produce IFN-g, express the T-box transcription factors (TFs) Eomes and/or T-bet, and are positive for the cytotoxicity receptors NK1.1 and NKp46. This population is composed of at least two cell types, NK cells and ILC1, which are distinguished by their developmental origins and requirements. The mouse salivary glands (SG) contain a large population of NK1.1 + cells which are IL-15 dependent but only minimally produce IFN-g. Our studies reveal that NK1.1 + cells in the SG represent a novel population of tissue resident Eomes + ILC1 (Chapter 2). SG ILC1 expressed markers indicative of tissue residency and have alternative functionality through the expression of the death inducing ligand TRAIL as well as CD39 and CD73 which can generate adenosine from ATP. SG ILC1 had distinct requirements for the TFs NFIL3, Eomes, and T-bet compared to both NK cells and Eomes - ILC1. Whole genome microarray confirmed that SG ILC1 were a separate population in addition to revealing that they expressed genes found in both NK cells and Eomes - ILC1. We also found that the distinguishing properties of SG ILC1 were dependent on TGF-b (Chapter 3). Loss of TGF-b signaling resulted in SG ILC1 becoming phenotypically and functionally similar to NK cells while TGF-b exposure caused NK cells to upregulate SG ILC1 markers. Mechanistically TGF-b repressed the expression of Eomes which acted to promote NK cell markers and limit TGF-b imprinting. TGF-b acted through a non-canonical Smad4-independent pathway which partially depended on JNK signaling. TGF-b mediated SG ILC1 differentiation occurred in an age dependent manner which mirrored SG development. Human SG also harbor a cell population which express markers reminiscent of mouse SG ILC1. Taken together, these studies have expanded the group 1 ILC family to include the TGF-b imprinted, Eomes + SG ILC1.
Language
English (en)
Chair and Committee
Marco Colonna
Committee Members
Wayne Yokoyama, Thad Stappenbeck, Tony French, Gene Oltz,
Recommended Citation
Cortez, Victor Samuel, "Characterization of Salivary Gland Innate Lymphoid Cells" (2016). Arts & Sciences Electronic Theses and Dissertations. 740.
https://openscholarship.wustl.edu/art_sci_etds/740
Included in
Allergy and Immunology Commons, Immunology and Infectious Disease Commons, Medical Immunology Commons
Comments
Permanent URL: https://doi.org/10.7936/K7Z036FC